spinning disk fluorescent microscopy image system (Evident Corporation)
90
Structured Review
Evident Corporation
spinning disk fluorescent microscopy image system
Spinning Disk Fluorescent Microscopy Image System, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spinning+disk+fluorescent+microscopy+image+system/spinning+disk+fluorescent+microscopy+image+system/pmc04048743-313-31-30
Average 90 stars, based on 1 article reviews
Spinning Disk Fluorescent Microscopy Image System, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spinning+disk+fluorescent+microscopy+image+system/spinning+disk+fluorescent+microscopy+image+system/pmc04048743-313-31-30
Average 90 stars, based on 1 article reviews
spinning disk fluorescent microscopy image system - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Involvement of ERK2 in traumatic spinal cord injury"
Article Title: Involvement of ERK2 in traumatic spinal cord injury
Journal: Journal of neurochemistry
doi: 10.1111/j.1471-4159.2010.06579.x
Figure Legend Snippet: Silencing effects of pERK2 and caspase 3 with siRNA after excitotoxic SCI. (A, B, and C): Intrathecal pre-treatment for 72 h with ERK2 siRNA (B and C light bar, 2.5 μg) complexed with lipofectamine 2000 (5 μg) resulted in significant decrease in pERK2 protein expression 4 h after excitotoxic SCI, compared to control siRNA pretreatment (A and C dark bar, 2.5 μg). (D, E, and F): Intrathecal pre-treatment for 72 h with ERK2 siRNA (E and F light bar, 2.5 μg) complexed with lipofectamine 2000 (5 μg) resulted in significant decrease in caspase 3 activity (cleaved caspase 3) 4 h after excitotoxic SCI, compared to control siRNA pretreatment (D and F dark bar, 2.5 μg). The fluorescent labeled pERK2-positive or cleaved caspase 3-positive cells in the QUIS injection epicenter were counted using fliorescent microscopy image system (n=3 sections sparated 60 μm apart per animal, n=3 animals per group). Data were presented as mean ± S.E.M. and analyzed by t-test **p<0.01 and ***p<0.001, t test. Scale bar: 30 μm.
Techniques Used: Expressing, Activity Assay, Labeling, Injection, Microscopy
Related Articles
Plasmid Preparation:Article Title: Involvement of ERK2 in traumatic spinal cord injury Article Snippet: Briefly, sections were immunolabeled with polyclonal antibodies against ERK2, pERK2 (1:200, Cell Signaling), cleaved caspase 3 (1:200, Cell Signaling) or ED-1 (1:200, Chemicon, Temecula, CA) followed by incubation with Alexa Fluor 594 or FITC-conjugated secondary antibodies. .. The fluorescent ERK2 or ED-1signal in the lentiviral vector injection epicenter were imaged/analyzed and the pERK2-positive or cleaved caspase 3-positive cells in the QUIS lesion epicenter were counted using an Olympus Injection:Article Title: Involvement of ERK2 in traumatic spinal cord injury Article Snippet: Briefly, sections were immunolabeled with polyclonal antibodies against ERK2, pERK2 (1:200, Cell Signaling), cleaved caspase 3 (1:200, Cell Signaling) or ED-1 (1:200, Chemicon, Temecula, CA) followed by incubation with Alexa Fluor 594 or FITC-conjugated secondary antibodies. .. The fluorescent ERK2 or ED-1signal in the lentiviral vector injection epicenter were imaged/analyzed and the pERK2-positive or cleaved caspase 3-positive cells in the QUIS lesion epicenter were counted using an Olympus Microscopy:Article Title: Involvement of ERK2 in traumatic spinal cord injury Article Snippet: Briefly, sections were immunolabeled with polyclonal antibodies against ERK2, pERK2 (1:200, Cell Signaling), cleaved caspase 3 (1:200, Cell Signaling) or ED-1 (1:200, Chemicon, Temecula, CA) followed by incubation with Alexa Fluor 594 or FITC-conjugated secondary antibodies. .. The fluorescent ERK2 or ED-1signal in the lentiviral vector injection epicenter were imaged/analyzed and the pERK2-positive or cleaved caspase 3-positive cells in the QUIS lesion epicenter were counted using an Olympus Software:Article Title: Involvement of ERK2 in traumatic spinal cord injury Article Snippet: Briefly, sections were immunolabeled with polyclonal antibodies against ERK2, pERK2 (1:200, Cell Signaling), cleaved caspase 3 (1:200, Cell Signaling) or ED-1 (1:200, Chemicon, Temecula, CA) followed by incubation with Alexa Fluor 594 or FITC-conjugated secondary antibodies. .. The fluorescent ERK2 or ED-1signal in the lentiviral vector injection epicenter were imaged/analyzed and the pERK2-positive or cleaved caspase 3-positive cells in the QUIS lesion epicenter were counted using an Olympus |